WebSep 1, 2011 · Genomic DNA was removed by incubation with RNase-free DNase I (M0303S: New England BioLabs; Ipswich, MA, UK) in the presence of RNase inhibitor. The RNA was annealed with random hexamer and oligo-dt primers, and first strand synthesis was carried out with MuLV reverse transcriptase. WebDNase I, (RNase-free) is an endonuclease that nonspecifically cleaves DNA to release di-, tri- and oligonucleotide products with 5´-phosphorylated and 3´-hydroxylated …
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WebDNase I, (RNase-free) is an endonuclease that nonspecifically cleaves DNA to release di-, tri- and oligonucleotide products with 5´-phosphorylated and 3´-hydroxylated ends. … DNase I Reaction Buffer (10X) 10 μl (1X) DNAse I (RNase-free) 1 μl (2 units) … DNase I Reaction Buffer: B0303SVIAL-20 : 4 x 1.5 ml : 10 X : Properties & Usage. … Rna Synthesis Products - DNase I (RNase-free) NEB The OneTaq One-Step RT-PCR Kit offers sensitive and robust end-point detection … The Exo Selector has been designed to simplify the process of selecting the … In vitro synthesis of single-stranded RNA molecules is a widely used laboratory … Contact NEBsolutions - DNase I (RNase-free) NEB Restriction Endonucleases - DNase I (RNase-free) NEB WebAug 21, 2015 · After treatment with DNase I (catalog No. M0303S, New England Biolabs), the RNA concentration and the 260/280 nm absorbance ratio were assessed using a spectrophotometer (SpectraMax Plus, Molecular Devices). Total RNA (1 μg) was reverse-transcribed to cDNA using the Omniscript RT kit (catalog No. 205111, Qiagen). rice on shelves crossword
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WebAM2224 5X2,000U DNASE I (RNASE-FREE) (2 U/UL) (LABORATORY REAGENTS FOR RESEARCH PURPOSE) W1001-10 Zymo DNase/RNase-Free Water, 10ml [LAB … WebHighlights. Isolated from a recombinant source. Supplied with 10X Reaction Buffer DNase I, (RNase-free) is an endonuclease that nonspecifically cleaves DNA to release di-, tri- and … WebDec 20, 2024 · DNase I ( NEB #M0303) is inhibited by salt concentrations >50 mM. DNase I-XT is a salt-tolerant DNase I which retains activity up to 300 mM salt, but with optimal … redirect email to outlook